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Nrp1, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nrp1  (ABclonal Biotechnology)
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(A) Schematic illustration of Lung@DTX-Ad. Pep33 contains an <t>NRP1-binding</t> motif (CRGDK, red) and a lipid droplet-targeting sequence (AAM-B, green), and was incorporated during adiposome preparation by co-evaporation with DOPC. (B) Confocal images of cells transfecting EGFP or Pep33-EGFP. Lipid droplets were stained with LipidTOX Red. Scale bars: 10 μm. (C) Silver staining analysis of Pep33 associated with adiposomes after purification. (D-E) Physicochemical characterization of DTX-Ad with or without Pep33 modification, measured by DLS (n = 9). (F) Western blot and silver staining analysis of NRP1 expression in major organs. (G) Ex vivo fluorescence imaging of major organs collected 3 h after intravenous injection of saline, fluorescent adiposomes, or Lung@Ad into BALB/c mice, with quantification of the lung-to-liver signal ratio (n = 3 or 4). (H) Docetaxel concentrations in lung tissues measured by LC-MS/MS at 1 h after intravenous injection of the indicated formulations (n = 6). (I) Hemolysis assay of different formulations at the indicated concentrations. (J) Western blot analysis of NRP1 expression in different cancer cell lines. (K) Quantification of tumor burden in a lung metastasis model established by intravenous injection of 5 × 10 4 4T1-Luc cells into BALB/c mice, followed by treatment as indicated (n = 7 (L) Representative bioluminescence images of mice during treatment. Data are presented as mean ± SEM. Statistical differences were calculated using two-tailed Student’s t test. *** P <0.001, ** P <0.01, * P <0.05.
Nrp1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive selection macs separation kit  (Miltenyi Biotec)
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(A) Schematic illustration of Lung@DTX-Ad. Pep33 contains an <t>NRP1-binding</t> motif (CRGDK, red) and a lipid droplet-targeting sequence (AAM-B, green), and was incorporated during adiposome preparation by co-evaporation with DOPC. (B) Confocal images of cells transfecting EGFP or Pep33-EGFP. Lipid droplets were stained with LipidTOX Red. Scale bars: 10 μm. (C) Silver staining analysis of Pep33 associated with adiposomes after purification. (D-E) Physicochemical characterization of DTX-Ad with or without Pep33 modification, measured by DLS (n = 9). (F) Western blot and silver staining analysis of NRP1 expression in major organs. (G) Ex vivo fluorescence imaging of major organs collected 3 h after intravenous injection of saline, fluorescent adiposomes, or Lung@Ad into BALB/c mice, with quantification of the lung-to-liver signal ratio (n = 3 or 4). (H) Docetaxel concentrations in lung tissues measured by LC-MS/MS at 1 h after intravenous injection of the indicated formulations (n = 6). (I) Hemolysis assay of different formulations at the indicated concentrations. (J) Western blot analysis of NRP1 expression in different cancer cell lines. (K) Quantification of tumor burden in a lung metastasis model established by intravenous injection of 5 × 10 4 4T1-Luc cells into BALB/c mice, followed by treatment as indicated (n = 7 (L) Representative bioluminescence images of mice during treatment. Data are presented as mean ± SEM. Statistical differences were calculated using two-tailed Student’s t test. *** P <0.001, ** P <0.01, * P <0.05.
Positive Selection Macs Separation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd304 bdca 4 neuropilin 1 microbead kit  (Miltenyi Biotec)
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(A) Schematic illustration of Lung@DTX-Ad. Pep33 contains an <t>NRP1-binding</t> motif (CRGDK, red) and a lipid droplet-targeting sequence (AAM-B, green), and was incorporated during adiposome preparation by co-evaporation with DOPC. (B) Confocal images of cells transfecting EGFP or Pep33-EGFP. Lipid droplets were stained with LipidTOX Red. Scale bars: 10 μm. (C) Silver staining analysis of Pep33 associated with adiposomes after purification. (D-E) Physicochemical characterization of DTX-Ad with or without Pep33 modification, measured by DLS (n = 9). (F) Western blot and silver staining analysis of NRP1 expression in major organs. (G) Ex vivo fluorescence imaging of major organs collected 3 h after intravenous injection of saline, fluorescent adiposomes, or Lung@Ad into BALB/c mice, with quantification of the lung-to-liver signal ratio (n = 3 or 4). (H) Docetaxel concentrations in lung tissues measured by LC-MS/MS at 1 h after intravenous injection of the indicated formulations (n = 6). (I) Hemolysis assay of different formulations at the indicated concentrations. (J) Western blot analysis of NRP1 expression in different cancer cell lines. (K) Quantification of tumor burden in a lung metastasis model established by intravenous injection of 5 × 10 4 4T1-Luc cells into BALB/c mice, followed by treatment as indicated (n = 7 (L) Representative bioluminescence images of mice during treatment. Data are presented as mean ± SEM. Statistical differences were calculated using two-tailed Student’s t test. *** P <0.001, ** P <0.01, * P <0.05.
Cd304 Bdca 4 Neuropilin 1 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse rat neuropilin 1 blocking antibody  (R&D Systems)
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(A) Schematic illustration of Lung@DTX-Ad. Pep33 contains an <t>NRP1-binding</t> motif (CRGDK, red) and a lipid droplet-targeting sequence (AAM-B, green), and was incorporated during adiposome preparation by co-evaporation with DOPC. (B) Confocal images of cells transfecting EGFP or Pep33-EGFP. Lipid droplets were stained with LipidTOX Red. Scale bars: 10 μm. (C) Silver staining analysis of Pep33 associated with adiposomes after purification. (D-E) Physicochemical characterization of DTX-Ad with or without Pep33 modification, measured by DLS (n = 9). (F) Western blot and silver staining analysis of NRP1 expression in major organs. (G) Ex vivo fluorescence imaging of major organs collected 3 h after intravenous injection of saline, fluorescent adiposomes, or Lung@Ad into BALB/c mice, with quantification of the lung-to-liver signal ratio (n = 3 or 4). (H) Docetaxel concentrations in lung tissues measured by LC-MS/MS at 1 h after intravenous injection of the indicated formulations (n = 6). (I) Hemolysis assay of different formulations at the indicated concentrations. (J) Western blot analysis of NRP1 expression in different cancer cell lines. (K) Quantification of tumor burden in a lung metastasis model established by intravenous injection of 5 × 10 4 4T1-Luc cells into BALB/c mice, followed by treatment as indicated (n = 7 (L) Representative bioluminescence images of mice during treatment. Data are presented as mean ± SEM. Statistical differences were calculated using two-tailed Student’s t test. *** P <0.001, ** P <0.01, * P <0.05.
Mouse Rat Neuropilin 1 Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse polyclonal anti nrp1  (R&D Systems)
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(A) Schematic illustration of Lung@DTX-Ad. Pep33 contains an <t>NRP1-binding</t> motif (CRGDK, red) and a lipid droplet-targeting sequence (AAM-B, green), and was incorporated during adiposome preparation by co-evaporation with DOPC. (B) Confocal images of cells transfecting EGFP or Pep33-EGFP. Lipid droplets were stained with LipidTOX Red. Scale bars: 10 μm. (C) Silver staining analysis of Pep33 associated with adiposomes after purification. (D-E) Physicochemical characterization of DTX-Ad with or without Pep33 modification, measured by DLS (n = 9). (F) Western blot and silver staining analysis of NRP1 expression in major organs. (G) Ex vivo fluorescence imaging of major organs collected 3 h after intravenous injection of saline, fluorescent adiposomes, or Lung@Ad into BALB/c mice, with quantification of the lung-to-liver signal ratio (n = 3 or 4). (H) Docetaxel concentrations in lung tissues measured by LC-MS/MS at 1 h after intravenous injection of the indicated formulations (n = 6). (I) Hemolysis assay of different formulations at the indicated concentrations. (J) Western blot analysis of NRP1 expression in different cancer cell lines. (K) Quantification of tumor burden in a lung metastasis model established by intravenous injection of 5 × 10 4 4T1-Luc cells into BALB/c mice, followed by treatment as indicated (n = 7 (L) Representative bioluminescence images of mice during treatment. Data are presented as mean ± SEM. Statistical differences were calculated using two-tailed Student’s t test. *** P <0.001, ** P <0.01, * P <0.05.
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anti nrp1  (Cell Signaling Technology Inc)
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(A) Schematic illustration of Lung@DTX-Ad. Pep33 contains an <t>NRP1-binding</t> motif (CRGDK, red) and a lipid droplet-targeting sequence (AAM-B, green), and was incorporated during adiposome preparation by co-evaporation with DOPC. (B) Confocal images of cells transfecting EGFP or Pep33-EGFP. Lipid droplets were stained with LipidTOX Red. Scale bars: 10 μm. (C) Silver staining analysis of Pep33 associated with adiposomes after purification. (D-E) Physicochemical characterization of DTX-Ad with or without Pep33 modification, measured by DLS (n = 9). (F) Western blot and silver staining analysis of NRP1 expression in major organs. (G) Ex vivo fluorescence imaging of major organs collected 3 h after intravenous injection of saline, fluorescent adiposomes, or Lung@Ad into BALB/c mice, with quantification of the lung-to-liver signal ratio (n = 3 or 4). (H) Docetaxel concentrations in lung tissues measured by LC-MS/MS at 1 h after intravenous injection of the indicated formulations (n = 6). (I) Hemolysis assay of different formulations at the indicated concentrations. (J) Western blot analysis of NRP1 expression in different cancer cell lines. (K) Quantification of tumor burden in a lung metastasis model established by intravenous injection of 5 × 10 4 4T1-Luc cells into BALB/c mice, followed by treatment as indicated (n = 7 (L) Representative bioluminescence images of mice during treatment. Data are presented as mean ± SEM. Statistical differences were calculated using two-tailed Student’s t test. *** P <0.001, ** P <0.01, * P <0.05.
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human nrp 1 elisa kit  (Elabscience Biotechnology)
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(A) Schematic illustration of Lung@DTX-Ad. Pep33 contains an <t>NRP1-binding</t> motif (CRGDK, red) and a lipid droplet-targeting sequence (AAM-B, green), and was incorporated during adiposome preparation by co-evaporation with DOPC. (B) Confocal images of cells transfecting EGFP or Pep33-EGFP. Lipid droplets were stained with LipidTOX Red. Scale bars: 10 μm. (C) Silver staining analysis of Pep33 associated with adiposomes after purification. (D-E) Physicochemical characterization of DTX-Ad with or without Pep33 modification, measured by DLS (n = 9). (F) Western blot and silver staining analysis of NRP1 expression in major organs. (G) Ex vivo fluorescence imaging of major organs collected 3 h after intravenous injection of saline, fluorescent adiposomes, or Lung@Ad into BALB/c mice, with quantification of the lung-to-liver signal ratio (n = 3 or 4). (H) Docetaxel concentrations in lung tissues measured by LC-MS/MS at 1 h after intravenous injection of the indicated formulations (n = 6). (I) Hemolysis assay of different formulations at the indicated concentrations. (J) Western blot analysis of NRP1 expression in different cancer cell lines. (K) Quantification of tumor burden in a lung metastasis model established by intravenous injection of 5 × 10 4 4T1-Luc cells into BALB/c mice, followed by treatment as indicated (n = 7 (L) Representative bioluminescence images of mice during treatment. Data are presented as mean ± SEM. Statistical differences were calculated using two-tailed Student’s t test. *** P <0.001, ** P <0.01, * P <0.05.
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Image Search Results


(A) Schematic illustration of Lung@DTX-Ad. Pep33 contains an NRP1-binding motif (CRGDK, red) and a lipid droplet-targeting sequence (AAM-B, green), and was incorporated during adiposome preparation by co-evaporation with DOPC. (B) Confocal images of cells transfecting EGFP or Pep33-EGFP. Lipid droplets were stained with LipidTOX Red. Scale bars: 10 μm. (C) Silver staining analysis of Pep33 associated with adiposomes after purification. (D-E) Physicochemical characterization of DTX-Ad with or without Pep33 modification, measured by DLS (n = 9). (F) Western blot and silver staining analysis of NRP1 expression in major organs. (G) Ex vivo fluorescence imaging of major organs collected 3 h after intravenous injection of saline, fluorescent adiposomes, or Lung@Ad into BALB/c mice, with quantification of the lung-to-liver signal ratio (n = 3 or 4). (H) Docetaxel concentrations in lung tissues measured by LC-MS/MS at 1 h after intravenous injection of the indicated formulations (n = 6). (I) Hemolysis assay of different formulations at the indicated concentrations. (J) Western blot analysis of NRP1 expression in different cancer cell lines. (K) Quantification of tumor burden in a lung metastasis model established by intravenous injection of 5 × 10 4 4T1-Luc cells into BALB/c mice, followed by treatment as indicated (n = 7 (L) Representative bioluminescence images of mice during treatment. Data are presented as mean ± SEM. Statistical differences were calculated using two-tailed Student’s t test. *** P <0.001, ** P <0.01, * P <0.05.

Journal: bioRxiv

Article Title: Nature-inspired Nanoparticle Adiposomes Enable Targeted Delivery of Hydrophobic Drug for Anti-Cancer Treatment

doi: 10.64898/2026.06.01.729180

Figure Lengend Snippet: (A) Schematic illustration of Lung@DTX-Ad. Pep33 contains an NRP1-binding motif (CRGDK, red) and a lipid droplet-targeting sequence (AAM-B, green), and was incorporated during adiposome preparation by co-evaporation with DOPC. (B) Confocal images of cells transfecting EGFP or Pep33-EGFP. Lipid droplets were stained with LipidTOX Red. Scale bars: 10 μm. (C) Silver staining analysis of Pep33 associated with adiposomes after purification. (D-E) Physicochemical characterization of DTX-Ad with or without Pep33 modification, measured by DLS (n = 9). (F) Western blot and silver staining analysis of NRP1 expression in major organs. (G) Ex vivo fluorescence imaging of major organs collected 3 h after intravenous injection of saline, fluorescent adiposomes, or Lung@Ad into BALB/c mice, with quantification of the lung-to-liver signal ratio (n = 3 or 4). (H) Docetaxel concentrations in lung tissues measured by LC-MS/MS at 1 h after intravenous injection of the indicated formulations (n = 6). (I) Hemolysis assay of different formulations at the indicated concentrations. (J) Western blot analysis of NRP1 expression in different cancer cell lines. (K) Quantification of tumor burden in a lung metastasis model established by intravenous injection of 5 × 10 4 4T1-Luc cells into BALB/c mice, followed by treatment as indicated (n = 7 (L) Representative bioluminescence images of mice during treatment. Data are presented as mean ± SEM. Statistical differences were calculated using two-tailed Student’s t test. *** P <0.001, ** P <0.01, * P <0.05.

Article Snippet: The following primary antibodies were used in this study: β-Tubulin (Abclonal, #A12289, 1:5,000), β-Actin (Abclonal, #AC038, 1:10,000), GAPDH (Abclonal, #AC033, 1:50,000), GFP (Roche, #11814460001, 1:1,000), NRP1 (Abclonal, #A19087, 1:2,000), BCMA (ABMAX, #WPS001, 1:1,000), ApoE (Abclonal, #A0304SP, 1:2,000), and LDLR (Abclonal, #A14996SP, 1:1,000).

Techniques: Binding Assay, Sequencing, Evaporation, Staining, Silver Staining, Purification, Modification, Western Blot, Expressing, Ex Vivo, Fluorescence, Imaging, Injection, Saline, Liquid Chromatography with Mass Spectroscopy, Hemolysis Assay, Two Tailed Test